Interaction between nematode (Meloidogyne incognita) and Verticillium dahliae in cotton plant

Document Type : Research Paper

Authors

1 Associate Professor, Dept. of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources

2 MSc. student, Dept. of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources

3 Academic Member, Dept. of Plant Protection, Gorgan University of Paym-e-noor

4 Associate Professor of Cotton Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Gorgan, Iran

Abstract

Vascular wilt fungus, Verticillium dahliae, and root knot nematode, Meloidogyne incognita, are the most common and destructive pathogen factors in cotton. In order to study interactions between these two factors, their interaction effect on 4 cotton cultivars (Sahel, N200, Mehr and Varamin) was done in a completely randomized block design in 4 iterctions and 6 treatments in a greenhouse. Treatments including, control, just fungus, just nematode with concentration of 2300, just nematode with concentration of 7000, both fungus and nematode with concentration of 2300 and both fungus and nematode with concentration of 7000. 12×104 Microsclerote for each fungus treatment and 7000 and 2300 nematode eggs and second stage juvenile for the nematode treatments respectively (depending on the desired treatment) were inoculated to 60 days old cotton seedling. Meloidogyne incognita nematode was isolated from the host plant and then its species and race were determined and at last it was reproduced by inoculation to tomato transplants. After that the Verticillium dahliae fungus was isolated from infected cotton plants and after cultivation and identification steps, it was transfered to the Tajan wheat seed cultivar (three times been autoclaved) for reproducing.The results of this review after 2 months showed that the interactions between Verticillium dahliae fungus and Meloidogyne incognita nematode in cotton was resonator. As a combination of fungus and nematode simultaneously leads to a significant increase (P =%1) in the wiltdejection. With increment in nematode concentration, root rot, the number of nodes and egg mass increased and shoot and root length, fresh and dry weight decreased vice versa.

Keywords

References

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