Study of micromorphology, anatomical and ultrastructural morphology in two cotton varieties and their important application

Document Type : Research Paper

Authors

1 Assistant Profe., Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz Iran.

2 Cotton Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Gorgan, Iran.

3 Department of Genetic, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz Iran

Abstract

Anatomical and morphological study of seeds provide a wide range of features that play an important role in evaluation of seed quality and micromorphology identification of different genotypes. The health of constituent sections seed is always necessary for optimal germination and crop production. The aim of this research was to investigate the embryonic structure of seed in two cotton genotypes (Latif and Golestan) and importance of their application in cotton planting and improving yield. In this research the seeds of two genotypes were evaluated according to morphometric characteristics. The seed internal anatomy structure was studied after preparing and molding in paraffin, cutting with microtome, difference staining methods and finally viewing under light and fluorescent microscope. The results showed that seeds of Golestan genotype had more area surface, diameter, intra ventricular ratio, shape, spherical factors and 1000-seed weight. Meristems are more complete and Pro-meristems in Hypocotyl region are more developed in Golestan genotype than the Latif one. Mesophyll Chlorenchyma and Spongy cells have differentiated in two genotypes, but these cells had more growth in Golestan genotype. In Toluidine blue stain method which to identify the phenolic compounds, secretory structures were more chromatophilic in Latif variety than Golestan. Fluorescent microscope showed the present of abundant fluorescent compounds, in seed structure were seen under. Finally, we concluded that under same condition, Golestan genotype with rath maturity of embryo and more germination percent, have more seedling emergence than Latif genotype.

Keywords

References

  1. Agati, G. 1998. Response of the in vivo chlorophyll fluorescence spectrum to environmental factors and laser excitation wavelength. J Opt. A: Pure Appl. Opt. 7(4): 797–807.
  2. Alishah. O. 2014. Applied Instruction Planting, Caring and Harvesting of Cotton, Latif genotype. Ministry of Jihad–e-Agriculture Agricultural Research, Education & Extension Organization
  3. Alishah. O. and Miri, A. A.2010. Cotton Golestan genotype. Applied Instruction Ministry of Jihad–e-Agriculture Agricultural Research, Education & Extension Organization
  4. Ambika, S., Manonmani, V. and Somasundaram, G. 2014. Review on effect of seed size on seedling vigour and seed yield. Res J Seed Sci. 7: 31-38.
  5. Andersson, H., Baechi, T., Hoechl, M. and Richter, C. 1998. Autofluorescence of living cells. J. Micro. 191(1): 1–7
  6. Avci, U., Pattathil, S., Singh, B., Brown, V.L., Hahn, M.G., and Haigler, C.H. 2013. Cotton fiber cell walls of Gossypium hirsutum and Gossypium barbadense have differences related to loosely-bound xyloglucan. PLoS One, 8(2): 56315-56329.
  7. Bellaloui, N., Stetina, S.R., and Turley, R.B. 2015. Cottonseed protein, oil, and mineral status in near-isogenic Gossypium hirsutum cotton lines expressing fuzzy/linted and fuzzless/linted seed phenotypes under field conditions. Front Plant Sci. 19(6): 137.
  8. Benedict, J.C. 2015. A new technique to prepare hard fruits and seeds for anatomical studies. Appl Plant Sci. 3(10):1500075.
  9. Bentsink, L., and Koornneef, M. 2008. Seed dormancy and germination. The Arabidopsis book. American Society of Plant Biologists, 119 pp.
  10. Berlyn, G.M. and Miksche, J.P. 1976. Botanical microtechnique and cytochemistry., Ames, Iowa: The Iowa State University Press. 326 pp.
  11. Gadelha, I.C.N., Fonseca, N.B.S., Oloris, S.C.S., Melo, M.M., and Soto-Blanco, B. 2014. Gossypol toxicity from cottonseed products. Scientific World J. 2014: 231-635.
  12. He, Z., Zhang, H., and Olk, D.C. 2015. Chemical composition of defatted cottonseed and soy meal products. PLoS One, 10(6):129933.
  13. Horn, P.J., Korte, A.R., Neogi, P.B., Love, E., Fuchs, J., Strupat, K., and Chapman, K.D. 2012. Spatial mapping of lipids at cellular resolution in embryos of Cotton. Plant Cell, 24(2): 622-636.
  14. Paiziev, V.A.K. a. A.A. 2006. Structural features of the stony cotton seeds. NDT.net, 11(6): 7.
  15. Powell, A.A. 2010. Morphological and physiological characteristics of seeds and their capacity to germinate and survive. Ann Bot, 105(6): 975-976.
  16. Roshchina, V.V. 2007. Cellular models as biosensors. pp. 5–22. In V.V. Roshchina and S.S. Narwal, Eds., Cell diagnostics: Images, Biophysical and biochemical processes in allelopathy, Science, Enfield, UK.
  17. Roshchina, V.V. 2007. Luminescent cell analysis in allelopathy. pp. 103–115. In V.V. Roshchina and S.S. Narwal, (Eds.) Cell Diagnostics: Images, biophysical and biochemical Processes in Allelopathy. Science, Enfield, UK.
  18. Roshchina, V.V. 2012. Vital autofluorescence: application to the study of plant living cells. Inter J Spec. 2012:1-14.
  19. Roshchina, V.V., Yashin, V.A., Kononov, A.V. and Yashina, A.V. Laser-scanning confocal microscopy (LSCM): study of plant secretory cell. pp. 93–102. In V.V. Roshchina and S. S. Narwal. (Eds.) Cell Diagnostics: Images, biophysical and biochemical processes in allelopathy, Science, Enfield, UK.
  20. Roshchina, V.V., Yashina, A.V. and Yashin, V.A. 2008. Cell communication in pollen allelopathy analyzed with laser-scanning confocal microscopy. Allelopathy J. 21(2): 219–226.
  21. Roshchina, V.V., Yashina, A.V., Yashin, V.A. and Gol’tyaev, M.V. 2011. Fluorescence of biologically active compounds in plant secretory cells. pp. 3–25. In Narwal, S.S., Pavlovic, P. and John, J. (Eds.), Research Methods in Plant Science, Vol. 2. Forestry and Agroforestry, Studium Press, Houston, Tex, USA.
  22. Roshchina, V.V., Yashina, A.V., Yashin, V.A. and Prizova, N.K. 2009. Models to study pollen allelopathy. Allelopathy J. 23(1): 3–24.
  23. Ruan, Y.L., Llewellyn, D.J., and Furbank, R.T. 2003. Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. Plant Cell, 15(4): 952-964.
  24. Simpson, D.M.B., Adams, C.L., and Stone, G.M. 1940. Anatomical structure of the cottonseed coat as related to problems of germination: U.S. Department of Agriculture.
  25. Taylor, D.L. and Salmon, E.D. 1989. Basic fluorescence microscopy. pp. 207–237. In J.L. Wang and D.L. Taylor. (Eds.), Methods in cell biology: living cell in culture. Academic Press, San Diego, Calif, USA.
  26. Valdecasas, A.G. and Abad, A. 2011. Morphological confocal microscopy in arthropods and the enhancement of autofluorescence after proteinase K extraction. Microsc. Microanal. 17(1): 109–113.
  27. Vroh Bi, I., Baudoin, J.P., Hau, B., and Mergeai, G. 1999. Development of high-gossypol cotton plants with low-gossypol seeds using trispecies bridge crosses and in vitro culture of seed embryos. Euphytica, 106(3): 243-251.
  28. Wa., J. 1962. Botanical histochemistry: principles and practice. W. H. San Francisco: Freeman and Company.
  29. Wendel JF, Brubaker CL, Seelanan T. The origin and evolution of Gossypium. 2010. pp. 1–18. In: Stewart JM, Oosterhuis DM, Heitholt JJ, Mauney JR, editors. Physiology of Cotton. Netherlands: Springer.
  30. Wolfbeis, O.S. 1985. The fluorescence of organic natural products. pp. 167–370. In S.G. Schulman (Ed.) Molecular Luminescence Spectroscopy: Methods and Applications John Wiley & Sons, New York, NY, USA.

Zhong, S., Leong, J., Ye, W., Xu, P., Lin, S.H., Liu, J.Y., and and Lin, Y.C. 2013. (−)-Gossypol-enriched cottonseed oil Inhibits proliferation and Adipogenesis of human breast pre-adipocytes. Anticancer Res, 33(3): 949-955.

Iranian Journal of Cotton Researches
Volume 5, Issue 1 - Serial Number 9
September 2017
Pages 33-48

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