Hormetic effects of reduced concentrations of glyphosate on germination and seedling characteristics of cotton

Document Type : Research Paper

Authors

1 Agricultural Engineering Department, Minab Higher Education center, University of Hormozgan, Bandar abbas.

2 Isfahan University of Technology

10.22092/ijcr.2024.366297.1220

Abstract

Background and Objectives: Hormesis refers to the beneficial response to exposure to low levels of chemicals under otherwise adverse conditions. Certain herbicides can stimulate growth at reduced concentrations, making them promising agents for inducing hormesis. However, research on herbicide-induced stimulatory effects on germination indices is limited. This study aims to evaluate the impact of different glyphosate concentrations on the germination and seedling characteristics of cotton.
 Materials and Methods: To assess the effect of glyphosate on cotton germination and seedling development, two experiments were conducted in 2024. These experiments explored the hormetic effects of glyphosate by incorporating it into the growth medium and by seed priming with various glyphosate concentrations. Both experiments were designed as randomised complete block designs with three replications. Glyphosate was applied at six concentrations: 10, 20, 40, 80, 160, and 320 ppm, alongside a control. The traits measured included seedling length, fresh and dry weight, germination percentage, seed germination index, phytotoxicity percentage, tolerance index, and germination speed.
 
Results: The results indicated that the 10 ppm glyphosate treatment elicited hormetic effects on several traits. In the growth medium experiment, this treatment increased seedling length by 83% compared to the control. Similarly, in the seed priming experiment, seedling length was enhanced by 164% with the same concentration. The 10 ppm treatment also resulted in a 60% increase in fresh weight and a 120% increase in dry weight in the growth medium experiment, while in the seed priming experiment, fresh weight increased by 250% and dry weight by 315% compared to the control. Additionally, the 10 ppm treatment improved seed germination by 77% in the growth medium test and by approximately 89% in the seed priming test. Conversely, the 40 and 320 ppm treatments were associated with the most pronounced inhibitory effects on seedling length, fresh weight, dry weight, and germination in both experiments.
 Conclusion: The 10 ppm glyphosate treatment demonstrated the most favourable impact on the studied traits. These findings suggest that non-toxic concentrations of glyphosate can enhance seedling establishment and improve competitive ability against weeds through hormesis.

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Main Subjects

References

  1. Abbas, T., Nadeem, M.A., Tanveer, A., Zohaib, A., and Rasool, T. 2015. Glyphosate hormesis increases growth and yield of chickpea (Cicer arietinum l.). Pakistan Journal of Weed Science Research, 21(4): 533–542.
  2. Agathokleous, E., and Calabrese, E.J. 2019. Hormesis can enhance agricultural sustainability in a changing world. Global Food Security, 20: 150-155. https://doi.org/10.1016/j.gfs.2019.02.005
  3. Anunciato, V.M. 2018. Effects of glyphosate on the growth and reproduction of Digitaria insularis biotypes resistant or susceptible to this herbicide. Dissertation, Universidade Estadual Paulista.
  4. Asgari, A., and Ghafori, A. 2023. The effects of biochar on improving the tolerance to salinity stress and some growth traits in cotton. Iranian Journal of Cotton Researches, 11(1): 1-12. (in Persian with English Abstract).
  5. Ather Nadeem, M., Abbas, T., Tanveer, A., Maqbool, R., Zohaib, A., and Shehzad, M.A. 2017. Glyphosate hormesis in broad-leaved weeds: a challenge for weed management. Archives of Agronomy and Soil Science, 63(3): 344-351.
  6. Barbosa, A.P., Zucareli, C., Freiria, G.H., Gomes, G.R., Bazzo, J.H.B., and Takahashi, L.S.A. 2017. Low rates of glyphosate on the process germination and corn seedling development. Revista Brasileira de Milho e Sorgo, 16(2): 240–250. https://doi.org/10.18512/1980-6477/rbms.v16n2p240-250.
  7. Barrena, R., Casals, E., Colón, J., Font, X., Sáchez, A., and Puntes, V. 2009. Evaluation of the ecotoxicity of model nanoparticles. Chemosphere, (75): 850-857.
  8. Belz, R.G., and Duke, S.O. 2014. Herbicides and plant hormesis. Pest Management Science, 70: 698-707.
  9. Brito, I.P., Tropaldi, L., Carbonari, C.A., and Velini, E.D. 2018. Hormetic effects of glyphosate on plants. Pest Management Science, 74(5): 1064–1070. https://doi.org/10.1002/ ps.4523
  10. Cedergreen, N., Felby, C., Porter., J.R., and Streibig, J.C. 2009. Chemical stress can increase crop yield. Field Crops Research, 114: 54-57.
  11. Cesco, V.J.S., Krenchinski, F.H., Rodrigues, D.M., Alcántara-de la Cruz, R., Duke, S.O., Velini, E.D., and Carbonari, C.A. 2024. Glyphosate hormesis effects on the vegetative and reproductive development of glyphosate-susceptible and-resistant Conyza sumatrensis biotypes. Environmental Pollution, 345: 123504.
  12. Chen, L., Liu, L., Lu, B., Ma, T., Jiang, D., Li, J., Zhang, K., Sun, H., Zhang, Y., and Bai, Z. 2020. Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum). PLoS ONE, 15: 0228241.
  13. de Moraes, C.P., de Brito, I.P., Tropaldi, L., Carbonari, C.A., and Velini, E.D. 2020. Hormetic effect of glyphosate on Urochloa decumbens plants. Journal of Environmental Science and Health, Part B, 55: 376-381.
  14. Duke, S.O., Cedergreen, N., Velini, E.D., and Belz, R.G. 2006. Hormesis: is it an important factor in herbicide use and allelopathy? Outlooks on Pest Management, 17(1): 29-33.
  15. Duke, S.O., Lydon, J., Koskinen, W.C., Moorman, T.B., Chaney, R.L., and Hammerschmidt, R. 2012. Glyphosate effects on plant mineral nutrition, crop rhizosphere microbiota, and plant disease in glyphosate-resistant crops. Journal of Agricultural and Food Chemistry, 60: 10375e10397. http://dx.doi.org/10.1021/jf302436u.
  16. Egido, L.L.D., Toorop, P.E., and Lanfermeijer, F.C. 2018. Seed priming improves germination of Arabis alpina under thermo-inhibiting conditions. Seed Science and Technology, 46(2): 285-303.
  17. Gitti, D.D.C., Arf, O., Peron, I.B.G., Portugal, J.R., Corsini, D.C.D.C., and Rodrigues, R.A.F. 2011. Glyphosate as a growth regulator in upland rice. Pesqui Agropecu´ aria Trop, 41(4): 500–507. https://doi.org/10.5216/pat.v41i4.10160.
  18. Harris, D. 2006. Development and testing of on-farm seed priming. Natural Resource Charter Case Studies, 3: 129- 177.
  19. Hoseiny-Rad, M., and Jagannath, S. 2011. Effect of herbicide Imazethapyr (pursuit™) on chickpea seed germination. Archives of Phytopathology and Plant Protection, 44(3): 224-230.
  20. 1999. International rules for seed testing. Rules 1999.
  21. Khan, S., Zhou, J.L., Ren, L., and Mojiri, A. 2020. Effects of glyphosate on germination, photosynthesis and chloroplast morphology in tomato. Chemosphere, 258: 127350.
  22. Li, S., Wang, T., Guo, J., Dong, Y., Wang, Z., Gong, L., and Li, X. 2021. Polystyrene microplastics disturb the redox homeostasis, carbohydrate metabolism and phytohormone regulatory network in barley. Journal of Hazardous Materials, 415: 125614. https://doi.org/10.1016/j.jhazmat.2021.125614
  23. Mobli, A., Matloob, A., and Chauhan, B.S. 2020. Glyphosate-induced hormesis: impact on seedling growth and reproductive potential of common sowthistle (Sonchus oleraceus). Weed Science, 68(6): 605–611. https://doi.org/10.1017/wsc.2020.77
  24. Moulick, D., Ghosh, D., and Santra, S.C. 2016. Evaluation of effectiveness of seed priming with selenium in rice during germination under arsenic stress. Plant Physiology and Biochemistry, 109: 571-578.
  25. Moulick, D., Santra, S.C., and Ghosh, D. 2018. Seed priming with Se mitigates As-induced phytotoxicity in rice seedlings by enhancing essential micronutrient uptake and translocation and reducing as translocation. Environmental Science and Pollution Research, 25: 26978-26991.
  26. Nikoumaram, S., and Sepehri, A. 2024. The study of tolerance indices, germination and transfer efficiency of seed reserves in Foxtail Millet seedlings (Setaria italica) under cadmium and polyvinyl chloride stresses. Journal of Soil and Plant Interactions, 15: 33-50. (in Persian with English Abstract).
  27. Nouri, M., and Haddioui, A. 2021. Improving seed germination and seedling growth of Lepidium sativum with different priming methods under arsenic stress. Acta Ecologica Sinica, 41(1): 64-71.
  28. Patane, C., Saita, A., and Sortino, O. 2013. Comparative effects of salt and water stress on seed germination and early embryo growth in two cultivars of sweet sorghum. Journal of Agronomy and Crop Science, 199(1): 30-37.
  29. Pettigrew, W.T. 2008. The Effect of Higher Temperatures on Cotton Lint Yield Production and Fiber Quality. Crop Science, 48: 278–285.
  30. Piotrowicz-Cieslak, A.I., Adomas, B., and Michalczyk, D.J. 2010. Different glyphosate phytotoxicity of seeds and seedlings of selected plant species. Polish Journal of Environmental Studies, 19: 123-129.
  31. Reis, S., Pavia, I., Carvalho, A., Moutinho-Pereira, J., Correia, C., and Lima-Brito, J. 2018. Seed priming with iron and zinc in bread wheat: effects in germination, mitosis and grain yield. Protoplasma, 1–16. https://doi.org/10.1007/s00709-018-1222-4.
  32. Sen, A. and Puthur, J.T. 2020. Seed priming-induced physiochemical and molecular events in plants coupled to abiotic stress tolerance: An overview. Priming-mediated stress and cross-stress tolerance in crop plants, pp.303-316.
  33. Shakri, I., Yahya, J. and Tabatabai, D. Investigating germination and seedling growth indices of sorghum lines and cultivars under salinity stress conditions. Iran Seed Science and Technology, 5(2): 65-75.
  34. Soares, C., Pereira, R., Spormann, S., and Fidalgo, F. 2019. Is soil contamination by a glyphosate commercial formulation truly harmless to non-target plants? -evaluation of oxidative damage and antioxidant responses in tomato. Environmental Pollution, 247: 256-265.
  35. Tu, M., Hurd, C., Robison, R., and Randall, J.M. 2001. Glyphosate. Weed control methods handbook, the nature conservancy. http://tncweeds.ucdavis.edu/products/ handbook/14.Glyphosate.pdf
  36. Velini, E.D., Trindade, M.L., and Barberis, L.R.M. 2010. Growth regulation and other secondary effects of herbicides. Weed Science, 58(3): 351-354. https://doi.org/10.1614/WS-D-09-00028.1
  37. Zobiole, L.H.S., Bonini, E.A., de Oliveira, R.S., Kremer, R.J., and Ferrarese-Filho, O. 2010. Glyphosate affects lignin content and amino acid production in glyphosateresistant soybean. Acta Physiologiae Plantarum, 32: 831-837.

 

 

 

Iranian Journal of Cotton Researches
Volume 11, Issue 2
August 2023
Pages 117-131

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