پایش عامل بیماری سوختگی باکتریایی پنبه و بررسی باکتری‌های گرم منفی رورست در بذر پنبه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 استاد دانشکده کشاورزی دانشگاه بوعلی سینا همدان

چکیده

فیلوسفر مکانی برای زندگی ریزموجودات است. باکتری‌ها از جمله ساکنین فراوان فیلوسفر هستند که تعداد آنها تحت تاثیر گونه گیاه و نوع برگ قرار می‌گیرد. باکتری‌های متعددی بصورت رورست در سطح اندام‌های هوایی و بذور گیاه وجود داشته و در شرایط مساعد باعث بروز بیماری می‌شوند. در طی سال‌های اخیر با بروز همه‌گیری بیماری بلایت باکتریائی پنبه در کشور و بخصوص استان گلستان در سطح وسیع نظریاتی مبنی بر وجود رورستی باکتری در سطح بذور پنبه مطرح گردید. به منظور شناسائی باکتری‌های گرم منفی موجود در سطح بذور پنبه، ازبذور توصیه شده ارقام تجاری ساحل، ورامین، خرداد، بختگان و سپید بصورت تصادفی نمونه برداری شد. با قرار دادن بذور در داخل آب مقطر سترون و افزودن مقداری توئین 20 به عنوان ماده شوینده و قرار دادن محیط فوق در دمای اتاق به مدت یک ساعت به شستشوی بهتر باکتری‌های موجود در سطح بذور کمک شد. سوسپانسیون فوق در روی محیط کشت مناسب مخطط گردید. در ادامه باکتری‌های رشد کرده جداسازی، خالص‌سازی و بر اساس منابع معتبر باکتریولوژی شناسائی شدند. بر اساس نتایج حاصل گونه‌های Psuodomonas fluorescens، P. syringae، Pantoea annanas و Pectobacterium sp. شناسایی شدند. هیچ موردی از وجود باکتری Xanthomonas citri subsp.malvacearum به عنوان عامل بیماری بلایت باکتریایی به‌صورت رورست در بذور پنبه به اثبات نرسید.

کلیدواژه‌ها

عنوان مقاله [English]

The monitoring of causal agent of cotton bacterial blight and investigation of epiphytic gram negative bacteria on cotton seeds

نویسندگان [English]

  • Mohammad Razinataj 1
  • Gholam Khodakaramian 2
1 PhD. student, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2 Prof. Faculty of Agriculture- Bu-Ali Sina University
چکیده [English]

Phylosphere is a site for living microorganisms including bacteria. Plants species and plant leaf type have important role on diversity and density of bacteria live on them. There are several bacteria as epiphytic on aerial parts of plants and plant seeds which cause diseases in suitable conditions. Cotton bacterial blight has been appeared as epidemic in Iran particularly in Golestan province in recent years. Some suggestion propounds the presence of this bacterium on cotton seed surface. This study was conducted in Golestan province to identify the gram negative bacteria on seed surface. For this purpose samplings were done randomly from cotton cultivar seeds Sahel, Varamin, Bakhtegan, Sepid and Khordad for planting. The seeds were incorporated into sterile distilled water and small amount of tween 20 as a detergent was added to it, and put to room temperature for one hour for better washing of bacteria on seed surface. Then the above suspension was streaked on bacterial culture media. The bacteria were isolated, purified and identified. The results revealed that Pseudomonas fluorescens, P. syringae, Pantoea annanas and Pectobacterium sp. were identified based on authentic references. The presence of Xanthomonas citri subsp. malvacearum was not proved as causal agent of bacterial blight as epiphyte on cotton seeds.

کلیدواژه‌ها [English]

  • Cotton
  • bacterial blight
  • Xanthomonas citri subsp. malvacearum
  • Epiphyte
  • seed

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